RNA Details
Disease Name
pulmonary hypertension
Tissue
lung
RNA Symbol
Pdlim5
RNA ID
RNA Type
mRNA
Alteration Pattern
down regulation
Species
mus musculus
Detection Methods
Isolation of Mouse PASMC; Lentivirus-based miR-17?92 Overexpression in HPASMC; PDLIM5 3'-Untranslated Region Luciferase Reporter Assay
Target
Pdlim5?
Pathway
NA
PubMed ID
Title
Loss of MicroRNA-17 92 in Smooth Muscle Cells Attenuates Experimental Pulmonary Hypertension via Induction of PDZ and LIM Domain 5
Year
2015
Function
"Human PASMC (HPASMC) from patients with PAH expressed decreased levels of the miR-17 92 cluster, TGF-B, and SMC markers. Knockdown of Smad3 but not Smad2 prevented miR-17 92-induced expression of SMC markers. SMC-specific knockout of miR-17 92 attenuated hypoxia-induced pulmonary hypertension (PH) in mice, whereas reconstitution of miR-17 92 restored hypoxia-induced PH in these mice. PDLIM5 is a direct target of miR-17/20a, and hypertensive HPASMC and mouse PASMC expressed elevated PDLIM5 levels. Suppression of PDLIM5 increased expression of SMC markers and enhanced TGF-B/Smad2/3 activity in vitro and enhanced hypoxia-induced PH in vivo, whereas overexpression of PDLIM5 attenuated hypoxia-induced PH."